Advances in superresolution optical fluctuation imaging (SOFI)

Q Rev Biophys. 2013 May;46(2):210-21. doi: 10.1017/S0033583513000036. Epub 2013 May 14.

Abstract

We review the concept of superresolution optical fluctuation imaging (SOFI), discuss its attributes and trade-offs (in comparison with other superresolution methods), and present superresolved images taken on samples stained with quantum dots, organic dyes, and plasmonic metal nanoparticles. We also discuss the prospects of SOFI for live cell superresolution imaging and for imaging with other (non-fluorescent) contrasts.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Fluorescent Dyes / chemistry
  • Metal Nanoparticles / chemistry
  • Microscopy, Fluorescence / methods*
  • Optical Phenomena*
  • Organic Chemicals / chemistry

Substances

  • Fluorescent Dyes
  • Organic Chemicals