A protein phosphatase 2A complex spatially controls plant cell division

Nat Commun. 2013;4:1863. doi: 10.1038/ncomms2831.

Abstract

In the absence of cell migration, the orientation of cell divisions is crucial for body plan determination in plants. The position of the division plane in plant cells is set up premitotically via a transient cytoskeletal array, the preprophase band, which precisely delineates the cortical plane of division. Here we describe a protein complex that targets protein phosphatase 2A activity to microtubules, regulating the transition from the interphase to the premitotic microtubule array. This complex, which comprises TONNEAU1 and a PP2A heterotrimeric holoenzyme with FASS as regulatory subunit, is recruited to the cytoskeleton via the TONNEAU1-recruiting motif family of proteins. Despite the acentrosomal nature of plant cells, all members of this complex share similarity with animal centrosomal proteins involved in ciliary and centriolar/centrosomal functions, revealing an evolutionary link between the cortical cytoskeleton of plant cells and microtubule organizers in other eukaryotes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Arabidopsis / cytology*
  • Arabidopsis / enzymology*
  • Arabidopsis / ultrastructure
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Cell Division*
  • Germination
  • Isoenzymes / metabolism
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism*
  • Microtubules / metabolism
  • Multiprotein Complexes / metabolism*
  • Mutation / genetics
  • Phenotype
  • Phosphoprotein Phosphatases / metabolism
  • Plant Cells / enzymology*
  • Prophase
  • Protein Binding
  • Protein Interaction Maps
  • Protein Phosphatase 2 / genetics
  • Protein Phosphatase 2 / metabolism*
  • Seedlings / ultrastructure

Substances

  • Arabidopsis Proteins
  • FASS protein, Arabidopsis
  • Isoenzymes
  • Microtubule-Associated Proteins
  • Multiprotein Complexes
  • tonneau1 protein, Arabidopsis
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 2