Effects of resveratrol on bone mineral density in ovarectomized rats

Int J Biomed Sci. 2005 Jun;1(1):76-81.

Abstract

Hormone replacement therapy (HRT) has been used to prevent osteoporosis in postmenopausal women. However, HRT is not for everyone, due to concerns of side effects as well as increased risk of breast and possibly uterine cancer. Therefore, Dietary alternatives are considered, which include Trans-3,5,4'-Trihydroxystilbene (trans-resveratrol), a phytoestrogen naturally found in grapes, peanuts and wine with beneficial effects in both cardioprotective and chemopreventive. The purpose of this study was to evaluate the effects of trans-resveratrol on the bone metabolism in ovariectomized rats. 48 Rats were assigned to the following groups: sham surgery + normal diet; ovariectomy (Ovx) + normal diet; Ovx + diethylstilbestrol 0.03 mg × kgbw(-1) × d(-1);Ovx +Trans-Resveratrol 5 mg × kgbw(-1) × d(-1); Ovx + Trans-Resveratrol 15 mg × kgbw(-1) × d(-1); Trans-Resveratrol 45 mg × kgbw(-1) × d(-1). The rats were fed for 90 days. In the 90th day, OVX + Trans-Resveratrol 45 mg/(kgbw(-1)·d) group had a greater bone mineral density (BMD) than other groups. In the OVX + Trans-Resveratrol 45 mg/(kgbw(-1)·d), indices of endocortical bone formation (ALP 37.90 ± 2.96U/100ml, BGP 1.27 ± 0.10 ng/ml) were greater than those of the other groups, while the index of endocortical bone absorption (TRAP 10.35 ± 1.72 U/L) were lower than those of the other groups. Histopathological examination showed that resveratrol had no endometrial hyperplasia adverse effect. All of these support that resveratroal may have positive effect on postmenopausal osteoporosis prevention.

Keywords: bone mineral density; ovarectomized rats; post menopausal osteoporosis; trans-resveratrol.