Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage

Microbiology. 2013 Jul;159(Pt 7):1487-1496. doi: 10.1099/mic.0.067280-0. Epub 2013 May 15.

Abstract

Pectobacterium carotovorum subsp. carotovorum is a well-known plant pathogen that causes severe soft rot disease in various crops, resulting in considerable economic loss. To identify pathogenicity-related factors, Chinese cabbage was inoculated with 5314 transposon mutants of P. carotovorum subsp. carotovorum Pcc21 derived using Tn5 transposon mutagenesis. A total of 35 reduced-virulence or avirulent mutants were isolated, and 14 loci were identified. The 14 loci could be functionally grouped into nutrient utilization (pyrD, purH, purD, leuA and serB), production of plant cell-wall-degrading enzymes (PCWDEs) (expI, expR and PCC21_023220), motility (flgA, fliA and flhB), biofilm formation (expI, expR and qseC), susceptibility to antibacterial plant chemicals (tolC) and unknown function (ECA2640). Among the 14 genes identified, qseC, tolC and PCC21_023220 are novel pathogenicity factors of P. carotovorum subsp. carotovorum involved in biofilm formation, phytochemical resistance and PCWDE production, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Biofilms / growth & development
  • Brassica / microbiology*
  • DNA Transposable Elements / genetics
  • Drug Resistance, Bacterial
  • Gene Expression Regulation, Bacterial*
  • Microbial Sensitivity Tests
  • Mutagenesis, Insertional
  • Pectobacterium carotovorum / drug effects
  • Pectobacterium carotovorum / genetics
  • Pectobacterium carotovorum / metabolism
  • Pectobacterium carotovorum / pathogenicity*
  • Plant Diseases / microbiology*
  • Virulence / genetics
  • Virulence Factors / genetics
  • Virulence Factors / metabolism*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • DNA Transposable Elements
  • Virulence Factors