CAPITO--a web server-based analysis and plotting tool for circular dichroism data

Bioinformatics. 2013 Jul 15;29(14):1750-7. doi: 10.1093/bioinformatics/btt278. Epub 2013 May 15.


Motivation: Circular dichroism (CD) spectroscopy is one of the most versatile tools to study protein folding and to validate the proper fold of purified proteins. Here, we aim to provide a readily accessible, user-friendly and platform-independent tool capable of analysing multiple CD datasets of virtually any format and returning results as high-quality graphical output to the user.

Results: CAPITO (CD Anaylsis and Plotting Tool) is a novel web server-based tool for analysing and plotting CD data. It allows reliable estimation of secondary structure content utilizing different approaches. CAPITO accepts multiple CD datasets and, hence, is well suited for a wide application range such as the analysis of temperature or pH-dependent (un)folding and the comparison of mutants.


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Supplementary information: Supplementary data are available at Bioinformatics online.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Circular Dichroism / methods*
  • Internet
  • Protein Folding
  • Protein Structure, Secondary*
  • Software*