ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast

Elife. 2013 May 14;2:e00422. doi: 10.7554/eLife.00422.

Abstract

Mitochondrial division is important for mitochondrial distribution and function. Recent data have demonstrated that ER-mitochondria contacts mark mitochondrial division sites, but the molecular basis and functions of these contacts are not understood. Here we show that in yeast, the ER-mitochondria tethering complex, ERMES, and the highly conserved Miro GTPase, Gem1, are spatially and functionally linked to ER-associated mitochondrial division. Gem1 acts as a negative regulator of ER-mitochondria contacts, an activity required for the spatial resolution and distribution of newly generated mitochondrial tips following division. Previous data have demonstrated that ERMES localizes with a subset of actively replicating mitochondrial nucleoids. We show that mitochondrial division is spatially linked to nucleoids and that a majority of these nucleoids segregate prior to division, resulting in their distribution into newly generated tips in the mitochondrial network. Thus, we postulate that ER-associated division serves to link the distribution of mitochondria and mitochondrial nucleoids in cells. DOI:http://dx.doi.org/10.7554/eLife.00422.001.

Keywords: ERMES; Gem1; Miro; S. cerevisiae; mitochondria; mitochondrial DNA.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • DNA, Fungal / metabolism*
  • DNA, Mitochondrial / metabolism*
  • Endoplasmic Reticulum / metabolism*
  • Mitochondria / metabolism*
  • Mitochondrial Dynamics*
  • Multiprotein Complexes
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Signal Transduction

Substances

  • DNA, Fungal
  • DNA, Mitochondrial
  • GEM1 protein, S cerevisiae
  • Multiprotein Complexes
  • Saccharomyces cerevisiae Proteins