Comprehensive proteomic analysis of Trypanosoma cruzi epimastigote cell surface proteins by two complementary methods

J Proteome Res. 2013 Jul 5;12(7):3255-63. doi: 10.1021/pr400110h. Epub 2013 Jun 6.


Trypanosoma cruzi is a protozoan that causes Chagas' disease, a neglected infectious illness that affects millions of people, mostly in Latin America. Here, the cell surface subproteome of the T. cruzi epimastigote life form was characterized. In order to prepare samples enriched in epimastigote plasma membrane protein, two distinct methodologies were optimized and evaluated. The first methodology was based on cell surface trypsinization (Shave) of intact living cells while the second approach used biotinylation of cell surface proteins followed by streptavidin affinity chromatography isolation of the labeled proteins. Both T. cruzi subproteomes were analyzed by LC-MS/MS. The results showed that the methodologies offered comprehensive and complementary information about the parasite's plasma membrane subproteome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotinylation
  • Cell Membrane / metabolism
  • Chagas Disease / diagnosis*
  • Chagas Disease / pathology
  • Humans
  • Membrane Proteins / isolation & purification*
  • Membrane Proteins / metabolism
  • Proteome / analysis*
  • Tandem Mass Spectrometry
  • Trypanosoma cruzi / metabolism*
  • Trypanosoma cruzi / pathogenicity
  • Trypsin / chemistry


  • Membrane Proteins
  • Proteome
  • Trypsin