Evaluation of bacteriophage therapy to control Clostridium difficile and toxin production in an in vitro human colon model system

Anaerobe. 2013 Aug;22:25-30. doi: 10.1016/j.anaerobe.2013.05.001. Epub 2013 May 17.

Abstract

Clostridium difficile is a leading cause of hospital-acquired diarrhoea and represents a major challenge for healthcare providers. Due to the decreasing efficacy and associated problems of antibiotic therapy there is a need for synergistic and alternative treatments. In this study we investigated the use of a specific bacteriophage, ΦCD27, in a human colon model of C. difficile infection. Our findings demonstrate a significant reduction in the burden of C. difficile cells and toxin production with phage treatment relative to an untreated control, with no detrimental effect on commensal bacterial populations. The results demonstrate the potential of phage therapy, and highlight the limitations of using phages that have lysogenic capacity.

Keywords: Bacteriophage therapy; Clostridium difficile; Human colon model; Lysogeny; Toxin.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / therapeutic use
  • Bacterial Toxins / antagonists & inhibitors*
  • Bacterial Toxins / biosynthesis*
  • Bacteriophages / drug effects*
  • Bacteriophages / growth & development
  • Biological Therapy / methods
  • Cells, Cultured
  • Clostridium Infections / drug therapy*
  • Clostridium Infections / microbiology
  • Clostridium difficile / metabolism
  • Clostridium difficile / pathogenicity*
  • Clostridium difficile / virology*
  • Colon / microbiology*
  • Humans
  • Lysogeny
  • Treatment Outcome

Substances

  • Anti-Bacterial Agents
  • Bacterial Toxins