Abstract
Five new phenylethanoid glycosides, cistanosides J-N (1-5), together with 15 known ones (6-20) were isolated from the stems of Cistanche deserticola cultured in Tarim desert, China. Their structures were elucidated on the basis of extensive spectroscopic analysis (IR, HR-ESIMS, 1D- and 2D-NMR) and chemical degradation. All the compounds obtained were examined for their inhibitory effect on the lipopolysaccharide (LPS)-induced nitric oxide (NO) production in mouse microglial cells (BV-2 cells), and compounds 2 and 8 showed potent inhibition on the NO production with IC50 values of 14.94 μM and 14.32 μM, respectively.
Keywords:
Anti-inflammatory activity; Cistanche deserticola; Cistanoside; Phenylethanoid glycoside.
Copyright © 2013 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology*
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Cell Line
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China
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Cistanche / chemistry*
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Ecosystem
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Glucosides / chemistry
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Glucosides / isolation & purification
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Glucosides / pharmacology*
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Glycosides / chemistry
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Glycosides / isolation & purification
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Glycosides / pharmacology*
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Inflammation / drug therapy
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Inflammation / metabolism*
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Inhibitory Concentration 50
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Lipopolysaccharides
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Mice
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Microglia / drug effects
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Microglia / metabolism
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Molecular Structure
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Nitric Oxide / biosynthesis*
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Phytotherapy
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
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Plant Stems / chemistry
Substances
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Anti-Inflammatory Agents
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Glucosides
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Glycosides
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Lipopolysaccharides
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Plant Extracts
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cistanoside K
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tubuloside B
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Nitric Oxide