Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins

Rabeka Alam; Joshua Zylstra; Danielle M Fontaine; Bruce R Branchini; Mathew M Maye

doi:10.1039/c3nr01842c

Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins

Nanoscale. 2013 Jun 21;5(12):5303-6. doi: 10.1039/c3nr01842c.

Authors

Rabeka Alam¹, Joshua Zylstra, Danielle M Fontaine, Bruce R Branchini, Mathew M Maye

Affiliation

¹ Department of Chemistry, Syracuse University, Syracuse, New York 13244, USA.

PMID: 23685756
DOI: 10.1039/c3nr01842c

Abstract

Sequential bioluminescence resonance energy transfer (BRET) and fluorescence resonance energy transfer (FRET) from firefly luciferase to red fluorescent proteins using quantum dot or rod acceptor/donor linkers is described. The effect of morphology and tuned optical properties on the efficiency of this unique BRET-FRET system was evaluated.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Fireflies / enzymology
Fluorescence Resonance Energy Transfer
Luciferases, Firefly / chemistry*
Luminescent Proteins / chemistry*
Quantum Dots*
Red Fluorescent Protein

Substances

Luminescent Proteins
Luciferases, Firefly