Novel Multistep BRET-FRET Energy Transfer Using Nanoconjugates of Firefly Proteins, Quantum Dots, and Red Fluorescent Proteins

Nanoscale. 2013 Jun 21;5(12):5303-6. doi: 10.1039/c3nr01842c.

Abstract

Sequential bioluminescence resonance energy transfer (BRET) and fluorescence resonance energy transfer (FRET) from firefly luciferase to red fluorescent proteins using quantum dot or rod acceptor/donor linkers is described. The effect of morphology and tuned optical properties on the efficiency of this unique BRET-FRET system was evaluated.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Fireflies / enzymology
  • Fluorescence Resonance Energy Transfer
  • Luciferases, Firefly / chemistry*
  • Luminescent Proteins / chemistry*
  • Quantum Dots*

Substances

  • Luminescent Proteins
  • red fluorescent protein
  • Luciferases, Firefly