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. 2012 Sep;9(3):164-7.
doi: 10.7497/j.issn.2095-3941.2012.03.002.

Effect of Coriolus Versicolor polysaccharide-B on the Biological Characteristics of Human Esophageal Carcinoma Cell Line eca109

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Free PMC article

Effect of Coriolus Versicolor polysaccharide-B on the Biological Characteristics of Human Esophageal Carcinoma Cell Line eca109

Dao-Feng Wang et al. Cancer Biol Med. .
Free PMC article

Abstract

Objective: To investigate the effect of Coriolus versicolor polysaccharide-B (CVPs-B) on the biological characteristics of human esophageal carcinoma cell line Eca109 in vitro.

Methods: The cells of experimental group (EG) were cultured in DMEM with 10% FCS and 150µg/mL CVPs-B, the cells of control group (CG) were cultured in DMEM with 10% FCS without CVPs-B. MTT reduction assay was performed to detect the effect of CVPs-B on the proliferation of Eca109 cells after the compound was administrated in varying concentrations. The living conditions of the Eca109 cells were determined using trypan blue exclusion. Then, cell growth curves were drawn. Flow cytometry was performed to detect the effect of CVPs-B on the apoptosis and cell cycle of Eca109.

Results: In comparison with the CG, a marked decrease in the proliferation of Eca09 cells was observed in the EG, after incubation with CVPs-B. The survival rate of Eca09 cells decreased as the time of CVPs-B incubation prolonged. Comparing the cell cycles and apoptotic rates between the two groups, the proportions of cells in the G0/G1, S, and G2/M phases in the EG were found to be (68.4±3.7)%, (13.9±2.1)%, and (17.7±1.4)%, respectively, after 24 h incubation with CVPs-B. The cells had an apoptotic rate of (9.7±0.7)%. On the other hand, the proportions of the G0/G1, S, and G2/M cells of the CG were found to be (53.9±3.6)%, (26.6±2.8)%, and (19.5±2.3)%, respectively, with an apoptotic rate of (5.7±1.4)%. In comparison with the CG cells, significant cell growth in the G0/G1 phase was observed in the EG (P<0.05). Furthermore, a significant decrease in the number of cells in the S phase was observed (P<0.05) in the EG.

Conclusions: CVPs-B can inhibit proliferation and enhance apoptosis of Eca109 cells and may be useful in the treatment of esophageal carcinoma.

Keywords: CVPs-B; apoptosis; esophageal carcinoma; proliferation.

Conflict of interest statement

No potential conflicts of interest are disclosed.

Figures

Figure 1
Figure 1
Effects of various concentrations of CVPs-B on the proliferation of esophageal cell line Eca109.
Figure 2
Figure 2
Changes in cell generation cycle and apoptosis of Eca109 cells in CG and EG.

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References

    1. Wang DF, Lou N, Zeng CG, et al. Expression of CXCL12 / CXCR4 and its correlation to prognosis in esophageal squamous cell carcinoma. Chinese Journal of Cancer 2009; 28: 154-158(in Chinese) - PubMed
    1. Lou N, Wang DF, Fang Y, et al. Effects of CVPs-B on macrophagocyte osteopontin-proteoglycan combined with oxidized low density lipoprotein. Chinese Pharmacological Bulletin 2009; 25: 1181-1184(in Chinese)
    1. Sasaki K, Natsugoe S, Ishigami S, et al. Expression of CXCL12 and its receptor CXCR4 correlates with lymph node metastasis in submucosal esophageal cancer. J Surg Oncol 2008; 97: 433-438 - PubMed
    1. Kaifi JT, Yekebas EF, Schurr P, et al. Tumor-cell homing to lymph nodes and bone marrow and CXCR4 expression in esophageal cancer. J Natl Cancer Inst 2005; 97: 1840-1847 - PubMed
    1. Wei M, Liang LZ, Zhang CQ, et al. Correlation of CXCR4/CXCL12 over- expression to lymph node metastasis and chronic inflammation in cervical adeno-carcinoma. Ai Zheng 2007; 26: 298-302(in Chinese) - PubMed

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