Osteogenic differentiation of adipose-derived stem cells prompted by low-intensity pulsed ultrasound

Cell Prolif. 2013 Jun;46(3):320-7. doi: 10.1111/cpr.12035.


Objectives: Based on in vivo studies, low-intensity pulsed ultrasound (LIPUS) stimulation has been widely used in the clinic for advancing bone growth during healing of non-union alignment, fractures and other osseous defects. In this study, we have investigated osteogenic differentiation of adipose stem cells (ASCs) regulated by LIPUS, and also in a preliminarily manner, we have discussed diverse effects of different duty ratio parameters.

Materials and methods: Mouse adipose stem cells were isolated and osteogenically induced. Then they were treated with LIPUS for 10 min/day for 3 days, 5 days and 7 days, respectively. Finally, effects of LIPUS on osteogenic differentiation of the ASCs were analysed by real-time PCR, western blotting and immunofluorescence.

Results: Our data indicated that LIPUS promoted mRNA levels of runt-related transcription factor 2, osteopontin and osterix in the presence of osteo-induction medium; moreover, protein levels of runt-related transcription factor 2 and osteopontin were upregulated.

Conclusions: We successfully demonstrated that LIPUS enhanced osteogenesis of ASCs, specially at the duty ratio of 20%.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology*
  • Adipose Tissue / cytology*
  • Animals
  • Bone and Bones / metabolism
  • Cell Differentiation*
  • Cell Proliferation
  • Cells, Cultured
  • Core Binding Factor Alpha 1 Subunit / genetics
  • Mesenchymal Stem Cells / cytology*
  • Mice
  • Osteogenesis*
  • Osteopontin / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sound*
  • Sp7 Transcription Factor
  • Transcription Factors / genetics
  • Up-Regulation


  • Core Binding Factor Alpha 1 Subunit
  • RNA, Messenger
  • Runx2 protein, mouse
  • Sp7 Transcription Factor
  • Sp7 protein, mouse
  • Transcription Factors
  • Osteopontin