A Kir6.2 pore mutation causes inactivation of ATP-sensitive potassium channels by disrupting PIP2-dependent gating

PLoS One. 2013 May 20;8(5):e63733. doi: 10.1371/journal.pone.0063733. Print 2013.

Abstract

In the absence of intracellular nucleotides, ATP-sensitive potassium (KATP) channels exhibit spontaneous activity via a phosphatidylinositol-4,5-bisphosphate (PIP2)-dependent gating process. Previous studies show that stability of this activity requires subunit-subunit interactions in the cytoplasmic domain of Kir6.2; selective mutagenesis and disease mutations at the subunit interface result in time-dependent channel inactivation. Here, we report that mutation of the central glycine in the pore-lining second transmembrane segment (TM2) to proline in Kir6.2 causes KATP channel inactivation. Unlike C-type inactivation, a consequence of selectivity filter closure, in many K(+) channels, the rate of inactivation in G156P channels was insensitive to changes in extracellular ion concentrations or ion species fluxing through the pore. Instead, the rate of G156P inactivation decreased with exogenous application of PIP2 and increased when PIP2-channel interaction was inhibited with neomycin or poly-L-lysine. These findings indicate the G156P mutation reduces the ability of PIP2 to stabilize the open state of KATP channels, similar to mutations in the cytoplasmic domain that produce inactivation. Consistent with this notion, when PIP2-dependent open state stability was substantially increased by addition of a second gain-of-function mutation, G156P inactivation was abolished. Importantly, bath application and removal of Mg(2+)-free ATP or a nonhydrolyzable analog of ATP, which binds to the cytoplasmic domain of Kir6.2 and causes channel closure, recover G156P channel from inactivation, indicating crosstalk between cytoplasmic and transmembrane domains. The G156P mutation provides mechanistic insight into the structural and functional interactions between the pore and cytoplasmic domains of Kir6.2 during gating.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Acyl Coenzyme A / physiology
  • Adenosine Triphosphate / physiology
  • Animals
  • Cell Line
  • Chlorocebus aethiops
  • Cricetinae
  • Hydrolysis
  • Ion Channel Gating
  • Membrane Potentials
  • Mutation, Missense*
  • Neomycin / pharmacology
  • Patch-Clamp Techniques
  • Phosphatidylinositol 4,5-Diphosphate / physiology*
  • Polylysine / pharmacology
  • Potassium Channels, Inwardly Rectifying / antagonists & inhibitors
  • Potassium Channels, Inwardly Rectifying / genetics*
  • Potassium Channels, Inwardly Rectifying / metabolism
  • Protein Stability
  • Rats
  • Sulfonylurea Receptors / metabolism

Substances

  • Acyl Coenzyme A
  • Kir6.2 channel
  • Phosphatidylinositol 4,5-Diphosphate
  • Potassium Channels, Inwardly Rectifying
  • Sulfonylurea Receptors
  • Polylysine
  • Adenosine Triphosphate
  • Neomycin