Inflammation marker, damage marker and anabolic hormone responses to resistance training with vascular restriction in older males

Clin Physiol Funct Imaging. 2013 Sep;33(5):393-9. doi: 10.1111/cpf.12044. Epub 2013 Apr 23.


The goal of this study was to examine anabolic hormone, muscle damage marker and inflammation marker responses to two types of resistance training protocols in older men. Thirty-six healthy older males (mean age = 56.6 ± 0.6 years) completed 6 weeks of high-intensity resistance training (HI-RT), low-intensity resistance training with vascular restriction (LI-BFR) or no exercise control group (CON) three times per week. Three upper body exercises were performed by both exercise groups at the same intensity (at 80% 1-RM), but lower body exercises were performed by the HI-RT group at 80% 1-RM and by the LI-BFR group at 20% 1-RM with vascular restriction. Resting serum creatine kinase (CK), interleukin 6 (IL-6), insulin-like growth factor-I (IGF-I), IGF binding protein 3 (IGFBP-3) and testosterone (T) were measured before and after training. No significant group differences in resting CK, IL-6, IGF-I, IGFBP-3 and T were detected following training (P>0.05). In addition, there were no significant changes in muscle cross-sectional area (CSA), but a trend for significant decreases in the percent changes in thigh subcutaneous fat (P = 0.051). Although training-induced anabolic hormone response did not reach statistical significance, our findings on CK and IL-6 indicated that the LI-BFR training protocol was safe and well tolerated for older men to perform to improve muscular strength.

Keywords: blood flow restriction; inflammation marker; muscle damage; resistance training; testosterone.

Publication types

  • Comparative Study

MeSH terms

  • Analysis of Variance
  • Biomarkers / blood
  • Creatine Kinase / blood
  • Humans
  • Inflammation Mediators / blood*
  • Insulin-Like Growth Factor Binding Protein 3 / blood
  • Insulin-Like Growth Factor I / metabolism
  • Interleukin-6 / blood
  • Lower Extremity / blood supply*
  • Male
  • Middle Aged
  • Muscle Contraction
  • Muscle Strength
  • Muscle, Skeletal / diagnostic imaging
  • Muscle, Skeletal / enzymology
  • Muscle, Skeletal / immunology
  • Muscle, Skeletal / metabolism*
  • Regional Blood Flow
  • Resistance Training* / adverse effects
  • Testosterone / blood*
  • Time Factors
  • Tomography, X-Ray Computed
  • Tourniquets* / adverse effects


  • Biomarkers
  • IGF1 protein, human
  • IGFBP3 protein, human
  • IL6 protein, human
  • Inflammation Mediators
  • Insulin-Like Growth Factor Binding Protein 3
  • Interleukin-6
  • Testosterone
  • Insulin-Like Growth Factor I
  • Creatine Kinase