AMP sensing by DEAD-box RNA helicases

J Mol Biol. 2013 Oct 23;425(20):3839-45. doi: 10.1016/j.jmb.2013.05.006. Epub 2013 May 20.

Abstract

In eukaryotes, cellular levels of adenosine monophosphate (AMP) signal the metabolic state of the cell. AMP concentrations increase significantly upon metabolic stress, such as glucose deprivation in yeast. Here, we show that several DEAD-box RNA helicases are sensitive to AMP, which is not produced during ATP hydrolysis by these enzymes. We find that AMP potently inhibits RNA binding and unwinding by the yeast DEAD-box helicases Ded1p, Mss116p, and eIF4A. However, the yeast DEAD-box helicases Sub2p and Dbp5p are not inhibited by AMP. Our observations identify a subset of DEAD-box helicases as enzymes with the capacity to directly link changes in AMP concentrations to RNA metabolism.

Keywords: AMP; ATPase; DEAD-box; RNA helicase; unwinding.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenosine Monophosphate / metabolism*
  • Adenosine Monophosphate / pharmacology
  • Adenosine Triphosphate / metabolism
  • DEAD-box RNA Helicases / chemistry
  • DEAD-box RNA Helicases / genetics
  • DEAD-box RNA Helicases / metabolism*
  • Energy Metabolism
  • Enzyme Activation / drug effects
  • Protein Binding / drug effects
  • RNA, Fungal / genetics
  • RNA, Fungal / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / metabolism
  • Thermodynamics

Substances

  • RNA, Fungal
  • Saccharomyces cerevisiae Proteins
  • Adenosine Monophosphate
  • Adenosine Triphosphate
  • DED1 protein, S cerevisiae
  • DEAD-box RNA Helicases