Genetic characterization of novel putative rhabdovirus and dsRNA virus from Japanese persimmon

J Gen Virol. 2013 Aug;94(Pt 8):1917-1921. doi: 10.1099/vir.0.054445-0. Epub 2013 May 23.

Abstract

Deep-sequencing analysis of nucleic acids from leaf tissue of Japanese persimmon trees exhibiting fruit apex disorder in some fruits detected two molecules that were graft transmitted to healthy seedlings. One of the complete genomes consisted of 13 467 nt and encoded six genes similar to those of plant rhabdoviruses. The virus formed a distinct cluster in the genus Cytorhabdovirus with lettuce necrotic yellows virus, lettuce yellow mottle virus and strawberry crinkle virus in a phylogenetic tree based on the L protein (RNA-dependent RNA polymerase, RdRp). The other consisted of 7475 nt and shared a genome organization similar to those of some insect and fungal viruses having dsRNA genomes. In a phylogenetic tree using the RdRp sequence of several unassigned dsRNA viruses, the virus formed a possible new genus cluster with two insect viruses, Circulifer tenellus virus 1 and Spissistilus festinus virus 1, and one plant virus, cucurbit yellows-associated virus.

MeSH terms

  • Cluster Analysis
  • Diospyros / virology*
  • High-Throughput Nucleotide Sequencing
  • Molecular Sequence Data
  • Phylogeny
  • Plant Diseases / virology
  • Plant Leaves / virology
  • RNA Viruses / classification*
  • RNA Viruses / genetics
  • RNA Viruses / isolation & purification*
  • RNA, Viral / genetics

Substances

  • RNA, Viral

Associated data

  • GENBANK/AB735628
  • GENBANK/AB735629