Effect of Beta-glucan on intestinal anastomoses in a rat model

Adv Clin Exp Med. Mar-Apr 2013;22(2):157-63.

Abstract

Objectives: The authors aimed to investigate the effect of β-Glucan on healing of an experimental left-sided colon anastomosis model.

Material and methods: Twenty adult male Wistar albino rats were randomized into two groups which had colonic transection and end-to-end anastomosis. Group I (Control): anastomosis group, received no treatment (n = 10); group II, anastomosis + β-Glucan (50 mg/kg/day within seven days after surgical procedure). Bursting pressure, hydroxyproline levels and histopathological characteristics of the anastomosis were analyzed.

Results: The average burst pressure of Groups I and II were 106.67 ± 5.00 and 148.00 ± 11.35 mmHg and hydroxyproline levels were 0.85 ± 0.14 and 1.45 ± 0.46 µg/mg, respectively. Both the burst pressure and hydroxyproline levels in group II were statistically significantly higher (p < 0.05). Histopathological examination revealed less epithelial damage in group II (p < 0.05). Though not statistically significant, less edema and damage to the submucosal-muscular layer was seen in Group II (p = 0079).

Conclusions: Due to significant increases in anastomotic bursting pressures and tissue hydroxyproline levels and considering the inhibitory effect of β-Glucan on epithelial damage, edema, and submucosal-muscular layer damage, β-Glucan was thought to contribute to the healing of the anastomosis.

MeSH terms

  • Anastomosis, Surgical / adverse effects
  • Animals
  • Colon / drug effects*
  • Colon / pathology
  • Colon / surgery
  • Disease Models, Animal
  • Drug Combinations
  • Hydrocortisone / metabolism
  • Male
  • Postoperative Complications / drug therapy*
  • Postoperative Complications / pathology
  • Postoperative Complications / physiopathology
  • Pressure
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Surgical Wound Dehiscence / drug therapy*
  • Surgical Wound Dehiscence / pathology
  • Surgical Wound Dehiscence / physiopathology
  • Urea / metabolism
  • Wound Healing / drug effects*
  • beta-Glucans / pharmacology*

Substances

  • Drug Combinations
  • beta-Glucans
  • hydrocortisone, urea drug combination
  • Urea
  • Hydrocortisone