A generic strategy for pharmacological caging of growth factors for tissue engineering

Chem Commun (Camb). 2013 Jul 7;49(53):5927-9. doi: 10.1039/c3cc41616j.

Abstract

The caging of small molecules has revolutionized biological research by providing a means to regulate a wide range of processes. Here we report on a generic pharmacological method to cage proteins in a similar fashion. The present approach is of value in both fundamental and applied research, e.g. in tissue engineering.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / chemistry
  • Animals
  • Cell Movement / drug effects
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • DNA Gyrase / chemistry
  • Fibroblasts / drug effects
  • Fibroblasts / physiology
  • HEK293 Cells
  • Human Umbilical Vein Endothelial Cells / drug effects
  • Human Umbilical Vein Endothelial Cells / physiology
  • Humans
  • Immunoglobulin Fc Fragments / chemistry
  • Immunoglobulin G / chemistry
  • Intercellular Signaling Peptides and Proteins / administration & dosage*
  • Intercellular Signaling Peptides and Proteins / chemistry*
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / physiology
  • Mice
  • Novobiocin / chemistry
  • Tissue Engineering*

Substances

  • Immunoglobulin Fc Fragments
  • Immunoglobulin G
  • Intercellular Signaling Peptides and Proteins
  • Novobiocin
  • Alkaline Phosphatase
  • DNA Gyrase