In vitro biocompatibility of Ti-45S5 bioglass nanocomposites and their scaffolds

J Biomed Mater Res A. 2014 May;102(5):1316-24. doi: 10.1002/jbm.a.34808. Epub 2013 Jun 7.


Titanium-10 wt % 45S5 Bioglass nanocomposites and their scaffolds were prepared by mechanical alloying (MA) followed by pressing, sintering, or combination of MA and a "space-holder" sintering process, respectively. An amorphous structure was obtained at 15 h of milling. The crystallization of the amorphous phase upon annealing led to the formation of a nanostructured Ti-10 wt % 45S5 Bioglass composite with a grain size of approximately 7 nm. The in vitro cytocompatibility of these materials was evaluated and compared with a conventional microcrystalline titanium. During the studies, established cell line of human fibroblasts CCD-39Lu was cultured in the presence of tested materials and its survival rate, and proliferation activity were examined. Furthermore, the influence of the Ti-45S5 Bioglass nanocomposites and microcrystalline titanium was tested on the growth of Candida albicans yeast. Biocompatibility tests carried out indicate that the nanocomposite Ti-10 wt % 45S5 Bioglass scaffolds could be a possible candidate for dental implants and other medicinal applications.

Keywords: bioglass; cell proliferation; cell viability; nanocomposite; scaffold.

MeSH terms

  • Biocompatible Materials / pharmacology*
  • Candida albicans / drug effects
  • Candida albicans / growth & development
  • Cell Death / drug effects
  • Cell Line
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Ceramics / pharmacology*
  • Colony Count, Microbial
  • Glass
  • Humans
  • Materials Testing*
  • Nanocomposites / chemistry*
  • Nanocomposites / ultrastructure
  • Propidium / metabolism
  • Tissue Scaffolds / chemistry*
  • Titanium / pharmacology*


  • Biocompatible Materials
  • bioactive glass 45S5
  • Propidium
  • Titanium