Advances in NMR structures of integral membrane proteins

Curr Opin Struct Biol. 2013 Aug;23(4):555-62. doi: 10.1016/ Epub 2013 May 27.


Integral membrane proteins (IMPs) play a central role in cell communication with the environment. Their structures are essential for our understanding of the molecular mechanisms of signaling and for drug design, yet they remain badly underrepresented in the protein structure databank. Solution NMR is, aside from X-ray crystallography, the major tool in structural biology. Here we review recently reported solution NMR structures of polytopic IMPs and discuss the new approaches, which were developed in the course of these studies to overcome barriers in the field. Advances in cell-free protein expression, combinatorial isotope labeling, resonance assignment, and collection of structural data greatly accelerated IMP structure determination by solution NMR. In addition, novel membrane-mimicking media made possible determination of solution NMR structures of IMPs in a native-like lipid environment.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cell Communication
  • Humans
  • Membrane Proteins / metabolism
  • Membrane Proteins / ultrastructure*
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Protein Structure, Tertiary
  • Signal Transduction


  • Membrane Proteins