Bacterial contamination of re-usable laryngoscope blades during the course of daily anaesthetic practice

S Afr Med J. 2013 Feb 19;103(6):386-9. doi: 10.7196/samj.6385.

Abstract

Background and objectives: Hospital-acquired infections (HAIs) are largely preventable through risk analysis and modification of practice. Anaesthetic practice plays a limited role in the prevention of HAIs, although laryngoscope use and decontamination is an area of concern. We aimed to assess the level of microbial contamination of re-usable laryngoscope blades at a public hospital in South Africa.

Setting: The theatre complex of a secondary-level public hospital in Johannesburg.

Methods: Blades from two different theatres were sampled twice daily, using a standardised technique, over a 2-week period. Samples were quantitatively assessed for microbial contamination, and stratified by area on blade, theatre and time using Fisher's exact test.

Results: A contamination rate of 57.3% (63/110) was found, with high-level contamination accounting for 22.2% of these. Common commensals were the most frequently isolated micro-organisms (79.1%), but important hospital pathogens such as Enterobacter species and Acinetobacter baumannii were isolated from blades with high-level contamination. No significant difference in the level of microbial contamination by area on blade, theatre or time was found (p<0.05).

Conclusions: A combination of sub-optimal decontamination and improper handling of laryngoscopes after decontamination results in significant microbial contamination of re-usable laryngoscope blades. There is an urgent need to review protocols and policies surrounding the use of these blades.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anesthesia*
  • Bacteria / isolation & purification*
  • Cross Infection / microbiology*
  • Cross Infection / prevention & control
  • Equipment Contamination*
  • Humans
  • Infection Control / methods
  • Laryngoscopes / microbiology*
  • Prospective Studies
  • South Africa