Fetal growth restriction caused by MIMT1 deletion alters brain transcriptome in cattle

Int J Dev Neurosci. 2013 Nov;31(7):463-7. doi: 10.1016/j.ijdevneu.2013.05.003. Epub 2013 May 29.

Abstract

We examined levels of gene expression in the brains of bovine fetuses carrying a truncated MIMT1 allele, MIMT1(Del), shown to cause late abortion and stillbirth as a result of fetal growth restriction. MIMT1 is a non-protein coding gene that forms part of the imprinted PEG3 (paternally expressed gene 3) domain. Microarray analysis of brain cortex samples from mid-gestation MIMT1(Del/WT) bovine fetuses and wild-type siblings was performed to study the effect of fetal growth restriction on brain gene expression. Statistical analysis revealed 134 genes with increased mRNA levels and 22 with reduced levels in MIMT1(Del/WT) fetuses. Gene set enrichment analysis identified a relatively small number of significant functional clusters representing three major biological processes: response to oxidative stress, angiogenesis, and epithelial cell proliferation. Gene expression microarray analyses identified increased expression of VIPR2, HTRA1, S100A4 and MYH8 in fetuses carrying the deletion and decreased expression of DRD2, ADAM18, miR345, ZNF585A. ADAM18, DRD2 and S100A4 are known to be involved in prenatal brain development. ZNF585A, miR-345, VIPR2, HTRA1, and MYH8 are known to be involved in cell growth and differentiation, but any role in neural developmental has yet to be elucidated.

Keywords: Bos taurus; Fetal brain; Intrauterine growth restriction; Non-coding RNA; Oxidative stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADAM Proteins / genetics
  • ADAM Proteins / metabolism
  • Animals
  • Brain / embryology*
  • Brain / metabolism*
  • Cattle
  • Cattle Diseases / genetics*
  • Cattle Diseases / pathology
  • Embryo, Mammalian
  • Female
  • Gene Expression Regulation, Developmental*
  • High-Temperature Requirement A Serine Peptidase 1
  • Kruppel-Like Transcription Factors / genetics*
  • Microarray Analysis
  • Myosin Heavy Chains / genetics
  • Myosin Heavy Chains / metabolism
  • Pregnancy
  • RNA, Messenger / metabolism
  • Receptors, Dopamine D2 / genetics
  • Receptors, Dopamine D2 / metabolism
  • Receptors, Vasoactive Intestinal Peptide, Type II / genetics
  • Receptors, Vasoactive Intestinal Peptide, Type II / metabolism
  • Serine Endopeptidases / genetics
  • Serine Endopeptidases / metabolism
  • Transcriptome / genetics*

Substances

  • Kruppel-Like Transcription Factors
  • RNA, Messenger
  • Receptors, Dopamine D2
  • Receptors, Vasoactive Intestinal Peptide, Type II
  • High-Temperature Requirement A Serine Peptidase 1
  • HTRA1 protein, human
  • Serine Endopeptidases
  • ADAM Proteins
  • Myosin Heavy Chains