Localization and function of KLF4 in cytoplasm of vascular smooth muscle cell

Biochem Biophys Res Commun. 2013 Jun 28;436(2):162-8. doi: 10.1016/j.bbrc.2013.05.067. Epub 2013 May 31.

Abstract

The Krüppel-like factor 4 is a DNA-binding transcriptional regulator that regulates a diverse array of cellular processes, including development, differentiation, proliferation, and apoptosis. The previous studies about KLF4 functions mainly focused on its role as a transcription factor, its functions in the cytoplasm are still unknown. In this study, we found that PDGF-BB could prompt the translocation of KLF4 to the cytoplasm through CRM1-mediated nuclear export pathway in vascular smooth muscle cells (VSMCs) and increased the interaction of KLF4 with actin in the cytoplasm. Further study showed that both KLF4 phosphorylation and SUMOylation induced by PDGF-BB participates in regulation of cytoskeletal organization by stabilizing the actin cytoskeleton in VSMCs. In conclusion, these results identify that KLF4 participates in the cytoskeletal organization by stabilizing cytoskeleton in the cytoplasm of VSMCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Cytoskeleton / drug effects
  • Actin Cytoskeleton / metabolism
  • Actins / metabolism
  • Active Transport, Cell Nucleus / drug effects
  • Animals
  • Becaplermin
  • Blotting, Western
  • Cell Nucleus / metabolism
  • Cells, Cultured
  • Cytoplasm / drug effects
  • Cytoplasm / metabolism*
  • HEK293 Cells
  • Humans
  • Karyopherins / metabolism
  • Kruppel-Like Factor 4
  • Kruppel-Like Transcription Factors / genetics
  • Kruppel-Like Transcription Factors / metabolism*
  • Male
  • Microscopy, Confocal
  • Muscle, Smooth, Vascular / cytology
  • Muscle, Smooth, Vascular / metabolism*
  • Myocytes, Smooth Muscle / drug effects
  • Myocytes, Smooth Muscle / metabolism*
  • Phosphorylation / drug effects
  • Protein Binding / drug effects
  • Proto-Oncogene Proteins c-sis / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Sumoylation / drug effects

Substances

  • Actins
  • KLF4 protein, human
  • Karyopherins
  • Klf4 protein, rat
  • Kruppel-Like Factor 4
  • Kruppel-Like Transcription Factors
  • Proto-Oncogene Proteins c-sis
  • Receptors, Cytoplasmic and Nuclear
  • exportin 1 protein
  • Becaplermin