Sialic acid-dependent attachment of mucins from three mouse strains to Entamoeba histolytica

Biochem Biophys Res Commun. 2013 Jun 28;436(2):252-8. doi: 10.1016/j.bbrc.2013.05.085. Epub 2013 May 31.

Abstract

Mouse strain-specific differences in the carbohydrate composition of intestinal mucins were hypothesized to account for strain-dependent susceptibility to Entamoeba histolytica. To test this hypothesis, intestinal mucins from susceptible and resistant inbred strains of mice were analyzed for their O-glycan content and for their ability to inhibit amoebic adherence to (GalNAc)12-27-HSA neo-glycoproteins. The results showed that the colorectal mucin O-glycan of susceptible CBA mice was lower in sialic acid content than that of resistant C57BL/6 and BALB/c mice. Mucins from CBA mice were more potent inhibitors of E. histolytica adherence to neo-glycoproteins than were mucins from C57BL/6 or BALB/c mice. Consistent with the role of terminal Gal/GalNAc as a receptor for amoebic adherence, sialidase treatment of C57BL/6 and BALB/c colorectal mucins increased their ability to inhibit E. histolytica adherence to the neo-glycoproteins. These results provide evidence of mouse strain-specific differences in the sialic acids content of mucin O-glycans. These dissimilarities likely contribute to the differential susceptibility of the three mouse strains to E. histolytica infection.

MeSH terms

  • Animals
  • Cell Membrane / metabolism
  • Colon / metabolism
  • Colon / parasitology
  • Entamoeba histolytica / metabolism*
  • Entamoeba histolytica / physiology
  • Glycomics / methods
  • Glycoproteins / metabolism
  • Host-Parasite Interactions / drug effects
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mucins / metabolism*
  • Mucins / pharmacology
  • N-Acetylneuraminic Acid / metabolism*
  • Polysaccharides / metabolism
  • Protein Binding / drug effects
  • Rectum / metabolism
  • Rectum / parasitology
  • Species Specificity

Substances

  • Glycoproteins
  • Mucins
  • Polysaccharides
  • N-Acetylneuraminic Acid