Caffeic acid phenethyl ester inhibits endothelial tissue factor expression

Biol Pharm Bull. 2013;36(6):1032-5. doi: 10.1248/bpb.b12-01039.


Caffeic acid phenethyl ester (CAPE) is a component of honeybee hives with various beneficial properties. Tissue factor (TF), the key trigger of thrombosis, is expressed in human endothelial cells. This study was designed to investigate whether CAPE modulates TF expression in human aortic endothelial cells (HAECs). Western blots and real-time polymerase chain reactions were performed. CAPE (10(-7)-10(-5) M) inhibited tumor necrosis factor (TNF)-α induced endothelial TF protein expression by 2.1-fold at 10(-5) M (p<0.0001). Similarly, TF surface activity was reduced (p<0.02). In contrast, TF mRNA expression, TF promoter activity, and mitogen-activated protein (MAP) kinase activation remained unaltered. In conclusion, CAPE inhibits TF protein expression and activity at the posttranscriptional level thereby exhibiting anti-thrombotic potential.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aorta / cytology
  • Caffeic Acids / pharmacology*
  • Cells, Cultured
  • Endothelial Cells / drug effects*
  • Endothelial Cells / metabolism
  • Fibrinolytic Agents / pharmacology*
  • Humans
  • Mitogen-Activated Protein Kinases / metabolism
  • Phenylethyl Alcohol / analogs & derivatives*
  • Phenylethyl Alcohol / pharmacology
  • RNA, Messenger / metabolism
  • Thromboplastin / antagonists & inhibitors*
  • Thromboplastin / genetics
  • Thromboplastin / metabolism
  • Tumor Necrosis Factor-alpha
  • Vascular Cell Adhesion Molecule-1 / genetics


  • Caffeic Acids
  • Fibrinolytic Agents
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • Vascular Cell Adhesion Molecule-1
  • Thromboplastin
  • Mitogen-Activated Protein Kinases
  • caffeic acid phenethyl ester
  • Phenylethyl Alcohol