Effect of Jobelyn® on intruder- and isolation-induced aggressive behavior in mice

J Basic Clin Physiol Pharmacol. 2013;24(4):263-9. doi: 10.1515/jbcpp-2012-0069.


Background: Aggression is a violent behavior emitted against another organism that may lead to its harm or death and thus is of public health significance, which necessitates the search for agents with anti-aggressive property. This study investigated the effect of Jobelyn® (JB), a unique African polyherbal formulation, on intruder- and isolation-induced aggressive behaviors in mice.

Methods: Male mice that showed aggression after being housed individually with female counterparts for 3 weeks or kept in isolation for 4 weeks were treated orally (p.o.) with JB (5, 10 or 50 mg/kg), haloperidol (HP) (1 mg/kg), fluoxetine (FL) (10 mg/kg), p-chlorophenylalanine (PCPA) (20 mg/kg), mianserin (MS) (50 mg/kg) or distilled water (10 mL/kg) 60 min before being tested for aggression. Interaction studies involving oral administration of PCPA (20 mg/kg), FL (10 mg/kg) or MS (50 mg/kg) to aggressive mice that had received JB (5 or 10 mg/kg, p.o.) 30 min earlier were assessed. The effect of JB (5, 10 or 50 mg/kg, p.o.) on defensive behaviors was also evaluated.

Results: JB (5, 10 or 50 mg/kg) decreased aggressive behaviors without impairing the defensive mechanisms of mice. PCPA (20 mg/kg), an inhibitor of 5-hydroxytryptamine (5-HT) biosynthesis, increased aggressive responses and reduced the anti-aggressive effect of JB. FL (10 mg/kg), a 5-HT reuptake inhibitor, significantly suppressed aggression but did not alter the effect of JB on aggression. MS (50 mg/kg), a 5-HT receptor antagonist, reduced aggression and enhanced the effect of JB on aggression.

Conclusions: These findings suggest that JB has anti-aggressive activity, which may be related to the enhancement of serotonergic system.

MeSH terms

  • Administration, Oral
  • Aggression / drug effects*
  • Animals
  • Behavior, Animal / drug effects*
  • Dietary Supplements
  • Dose-Response Relationship, Drug
  • Male
  • Medicine, African Traditional
  • Mice
  • Plant Preparations / administration & dosage
  • Plant Preparations / isolation & purification
  • Plant Preparations / pharmacology*
  • Serotonin Agents / administration & dosage
  • Serotonin Agents / isolation & purification
  • Serotonin Agents / pharmacology*
  • Social Isolation / psychology*


  • Plant Preparations
  • Serotonin Agents