Different expression of miRNAs targeting helicases in rice in response to low and high dose rate γ-ray treatments

Plant Signal Behav. 2013 Aug;8(8):e25128. doi: 10.4161/psb.25128. Epub 2013 Jul 8.

Abstract

Ionizing radiation currently represents an important tool to generate genetic variability that does not exist in nature, especially in plants. Even so, the radiological protection still represents a subject of regulatory concern. In plants, few reports dealing with the effects of γ-rays, in terms of dose rate (rate of energy deposition) and total dose (energy absorbed per unit mass), are available. In addition, plants are known to be more radioresistant than animals. The use of ionizing radiations for studying various aspects of transcription regulation may help elucidate some of the unanswered questions regarding DNA repair in plants. Under these premises, microRNAs have emerged as molecules involved in gene regulation in response to various environmental conditions as well as in other aspects of plant development. Currently, no report on the changes in microRNAs expression patterns in response to γ-ray treatments exists in plants, even if this subject is extensively studies in human cells. The present study deals with the expression profiles of three miRNAs, namely osa-miR414, osa-miR164e and osa-miR408 and their targeted helicase genes (OsABP, OsDBH and OsDSHCT) in response to different doses of γ-rays delivered both at low and high dose rates. The irradiated rice seeds were grown both in the presence of water and 100 mM NaCl solution. DNA damage and reactive species accumulation were registered, but no dose- or time-dependent expression was observed in response to these treatments.

Keywords: DNA damage; helicases; microRNAs; reactive oxygen species; relative expression; γ-ray.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Comet Assay
  • Computer Simulation
  • DNA Damage
  • DNA Helicases / metabolism*
  • Dose-Response Relationship, Radiation
  • Gamma Rays*
  • Gene Expression Profiling*
  • Gene Expression Regulation, Plant / drug effects
  • Gene Expression Regulation, Plant / radiation effects
  • Germination / drug effects
  • Germination / genetics
  • Germination / radiation effects
  • Humans
  • Hydrogen Peroxide / metabolism
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Oryza / enzymology
  • Oryza / genetics*
  • Oryza / radiation effects*
  • Salinity
  • Seedlings / drug effects
  • Seedlings / genetics
  • Seedlings / growth & development
  • Seedlings / radiation effects
  • Sodium Chloride / pharmacology
  • Stress, Physiological / drug effects
  • Stress, Physiological / genetics
  • Stress, Physiological / radiation effects

Substances

  • MicroRNAs
  • Sodium Chloride
  • Hydrogen Peroxide
  • DNA Helicases