The Hippo pathway polarizes the actin cytoskeleton during collective migration of Drosophila border cells

J Cell Biol. 2013 Jun 10;201(6):875-85. doi: 10.1083/jcb.201210073. Epub 2013 Jun 3.

Abstract

Collective migration of Drosophila border cells depends on a dynamic actin cytoskeleton that is highly polarized such that it concentrates around the outer rim of the migrating cluster of cells. How the actin cytoskeleton becomes polarized in these cells to enable collective movement remains unknown. Here we show that the Hippo signaling pathway links determinants of cell polarity to polarization of the actin cytoskeleton in border cells. Upstream Hippo pathway components localize to contacts between border cells inside the cluster and signal through the Hippo and Warts kinases to polarize actin and promote border cell migration. Phosphorylation of the transcriptional coactivator Yorkie (Yki)/YAP by Warts does not mediate the function of this pathway in promoting border cell migration, but rather provides negative feedback to limit the speed of migration. Instead, Warts phosphorylates and inhibits the actin regulator Ena to activate F-actin Capping protein activity on inner membranes and thereby restricts F-actin polymerization mainly to the outer rim of the migrating cluster.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Capping Proteins / metabolism
  • Actin Cytoskeleton / metabolism*
  • Actins / metabolism
  • Animals
  • Animals, Genetically Modified
  • Cell Movement / physiology*
  • Cell Polarity / physiology
  • DNA-Binding Proteins / metabolism
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / cytology*
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / metabolism
  • Feedback, Physiological / physiology
  • Female
  • Green Fluorescent Proteins / genetics
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Male
  • Nuclear Proteins / metabolism
  • Phosphorylation / physiology
  • Protein Kinases / metabolism
  • Protein-Serine-Threonine Kinases / metabolism*
  • Signal Transduction / physiology*
  • Trans-Activators / metabolism
  • Transgenes / physiology

Substances

  • Actin Capping Proteins
  • Actins
  • DNA-Binding Proteins
  • Drosophila Proteins
  • ENA-VASP proteins
  • Intracellular Signaling Peptides and Proteins
  • Nuclear Proteins
  • Trans-Activators
  • Yki protein, Drosophila
  • Green Fluorescent Proteins
  • Protein Kinases
  • wts protein, Drosophila
  • Protein-Serine-Threonine Kinases
  • hpo protein, Drosophila