15N-permethylated amino acids as efficient probes for MRI-DNP applications

Enrico Chiavazza; Alessandra Viale; Magnus Karlsson; Silvio Aime

doi:10.1002/cmmi.1538

15N-permethylated amino acids as efficient probes for MRI-DNP applications

Contrast Media Mol Imaging. 2013 Sep-Oct;8(5):417-21. doi: 10.1002/cmmi.1538.

Authors

Enrico Chiavazza¹, Alessandra Viale, Magnus Karlsson, Silvio Aime

Affiliation

¹ Department of Chemistry and Center for Molecular Imaging, University of Turin, Via Nizza 52, 10125 Torino, Italy.

PMID: 23740812
DOI: 10.1002/cmmi.1538

Abstract

The synthesis, NMR properties and preliminary polarization tests on protonated and perdeuterated forms of α-trimethylglutamine (NMe3Gln), α-trimethylglutamate (NMe3Glu) and ε-trimethyllysine (NMe3Lys) are reported. The (15)N-permethylated, perdeuterated amino acids display very long (15)N-T1 values, ranging between 190 and 330 s, are well polarized by the dynamic nuclear polarization (DNP) procedure, yielding good polarization levels (10%), and appear to be well tolerated by cells and mice. The obtained results make perdeuterated amino acids excellent candidates for innovative DNP (15)N-MRI applications such as perfusion or targeting studies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids / chemistry*
Animals
Cell Tracking*
Magnetic Resonance Imaging*
Mice
Nitrogen Isotopes / chemistry*
Staining and Labeling

Substances

Amino Acids
Nitrogen Isotopes