MicroRNA 181 suppresses porcine reproductive and respiratory syndrome virus (PRRSV) infection by targeting PRRSV receptor CD163

J Virol. 2013 Aug;87(15):8808-12. doi: 10.1128/JVI.00718-13. Epub 2013 Jun 5.


We previously showed that microRNA 181 (miR-181) can inhibit PRRSV replication by directly targeting its genomic RNA. Here, we report that miR-181 can downregulate the PRRSV receptor CD163 in blood monocytes and porcine alveolar macrophages (PAMs) through targeting the 3' untranslated region (UTR) of CD163 mRNA. Downregulation of CD163 leads to the inhibition of PRRSV entry into PAMs and subsequently suppresses PRRSV infection. Our findings indicate that delivery of miR-181 can be used as antiviral therapy against PRRSV infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / genetics
  • Antigens, Differentiation, Myelomonocytic / genetics
  • Macrophages, Alveolar / virology
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Monocytes / virology
  • Porcine respiratory and reproductive syndrome virus / growth & development*
  • Porcine respiratory and reproductive syndrome virus / physiology
  • Receptors, Cell Surface / antagonists & inhibitors*
  • Receptors, Cell Surface / genetics
  • Receptors, Virus / antagonists & inhibitors*
  • Receptors, Virus / genetics
  • Swine
  • Virus Internalization*


  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • CD163 antigen
  • MicroRNAs
  • Receptors, Cell Surface
  • Receptors, Virus