Abstract
It was reported that O glycosylation is associated with hepatic stellate cell activation and regulates collagen expression. In this study, we aimed to investigate the effect of O glycosylation on the activation of human hepatic stellate cells. We found that the inhibitor of O glycosylation, benzyl-α-GalNAc (2 and 4 mM), could significantly inhibit cells proliferation in a dose-dependent manner. Moreover, benzyl-α-GalNAc decreased the expressions of α-smooth muscle actin, collagen I, and collagen III. The results indicate that O glycosylation is involved in the activation of hepatic stellate cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylgalactosamine / analogs & derivatives*
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Acetylgalactosamine / pharmacology
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Actins / biosynthesis
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Benzyl Compounds / pharmacology*
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Cell Line
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Cell Proliferation / drug effects*
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Collagen Type I / biosynthesis
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Collagen Type III / biosynthesis
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Gene Expression / drug effects
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Glycosylation / drug effects*
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Hepatic Stellate Cells / drug effects
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Hepatic Stellate Cells / metabolism*
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Humans
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Liver / cytology
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Liver / drug effects
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Liver / metabolism*
Substances
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ACTA2 protein, human
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Actins
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Benzyl Compounds
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Collagen Type I
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Collagen Type III
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benzyl-alpha-N-acetylgalactosamine
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Acetylgalactosamine