Objective: To evaluate the ability of growth and proliferation of bladder urothelial cell and smooth muscle cell in 3-dimension (3-D) polyethylene glycol polyurethane (PEGPU) porous scaffolds.
Methods: The PEGPU scaffolds with 4 different pore size (90.8, 76.7, 40.9 and 39.6 microm) were prepared. Mouse fibroblasts, human bladder smooth muscle cells and human urothelial cells were implanted into the scaffolds and cultured respectively. The cell growth appearance on the outer and inner surface of 3-D scaffold was observed by FITC and DAPI dying, and the ability of growth and proliferation of implanted cells in 3-D scaffold was evaluated by CCK-8 method.
Results: All the three types of cell adhered on the surface and proliferated in the four different pore size 3-D scaffolds. Mouse fibroblasts, human bladder smooth muscle cells and human bladder urothelial cells achieved the optimum condition of growth and proliferation in the 3-D scaffold with mean pore size of 40.9 microm, 90.8 microm and 39.6 microm, respectively.
Conclusion: 3-D PEGPU scaffold shows the potential to be used in bladder tissue engineering.