A novel array format for monitoring cellular uptake using a photo-cleavable linker for peptide release

Kenji Usui; Takuya Kikuchi; Kin-ya Tomizaki; Takashi Kakiyama; Hisakazu Mihara

doi:10.1039/c3cc41632a

A novel array format for monitoring cellular uptake using a photo-cleavable linker for peptide release

Chem Commun (Camb). 2013 Jul 21;49(57):6394-6. doi: 10.1039/c3cc41632a.

Authors

Kenji Usui¹, Takuya Kikuchi, Kin-ya Tomizaki, Takashi Kakiyama, Hisakazu Mihara

Affiliation

¹ Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan. kusui@center.konan-u.ac.jp

PMID: 23749063
DOI: 10.1039/c3cc41632a

Abstract

We developed a novel peptide array format incorporating a photo-cleavable linker for monitoring cellular uptake. Model peptides were successfully immobilised via the photo-cleavable linker onto conventional plates and could be released spatiotemporally using UV irradiation. Incorporation of confocal microscopy allowed for detailed real-time monitoring of cellular internalisation of peptides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Transport
Cell-Penetrating Peptides / chemistry
Cell-Penetrating Peptides / metabolism*
Cross-Linking Reagents / chemistry
Cross-Linking Reagents / radiation effects
HeLa Cells
Humans
Immobilized Proteins / chemistry
Immobilized Proteins / metabolism*
Photochemical Processes
Ultraviolet Rays

Substances

Cell-Penetrating Peptides
Cross-Linking Reagents
Immobilized Proteins