Protocadherin-7 Induces Bone Metastasis of Breast Cancer

Biochem Biophys Res Commun. 2013 Jul 5;436(3):486-90. doi: 10.1016/j.bbrc.2013.05.131. Epub 2013 Jun 7.

Abstract

Breast cancer had a propensity to metastasize to bone, resulting in serious skeletal complications associated with poor outcome. Previous study showed that Protocadherin-7 (PCDH7) play an important role in brain metastatic breast cancer, however, the role of PCDH7 in bone metastatic breast cancer has never been explored. In the present study, we found that PCDH7 expression was up-regulation in bone metastatic breast cancer tissues by real-time PCR and immunohistochemistry assays. Furthermore, suppression of PCDH7 inhibits breast cancer cell proliferation, migration, and invasion in vitro by MTT, scratch, and transwell assays. Most importantly, overexpression of PCDH7 promotes breast cancer cell proliferation and invasion in vitro, and formation of bone metastasis in vivo. These data provide an important insight into the role of PCDH7 in bone metastasis of breast cancer.

Keywords: Bone metastasis; Breast cancer; PCHD7.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / metabolism
  • Bone Neoplasms / secondary*
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology*
  • Cadherins / genetics
  • Cadherins / metabolism*
  • Cell Line, Tumor
  • Cell Movement
  • Cell Proliferation
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Neoplasm Invasiveness / pathology
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism
  • Real-Time Polymerase Chain Reaction

Substances

  • Biomarkers
  • Cadherins
  • PCDH7 protein, human
  • RNA, Small Interfering