Regulation of cell adhesion and migration by cell-derived matrices

Exp Cell Res. 2013 Oct 1;319(16):2434-9. doi: 10.1016/j.yexcr.2013.05.030. Epub 2013 Jun 7.

Abstract

Three-dimensional in vitro extracellular matrix models provide a physiological alternative to regular two-dimensional cell culture, though they lack the full diversity of molecular composition and physical properties of whole-animal systems. Cell-derived matrices are extracellular matrices that are the product of matrix secretion and assembly by cells cultured at high density in vitro. After the removal of the cells that produced the matrix, an assembled matrix scaffold is left that closely mimics native stromal fiber organization and molecular content. Cell-derived matrices have been shown to impart in vivo-like responses to cells cultured in these matrices. In this review, we focus on mechanisms through which the distinct molecular and topographical composition of cell-derived matrices directs cellular behavior, specifically through regulation of cell-matrix adhesions and subsequent contributions to the process of cell migration.

Keywords: 3D cell migration; Cell-derived matrix (CDM); Extracellular matrix; Fibrillar topography; Matrix adhesions; Matrix elasticity.

Publication types

  • Research Support, N.I.H., Intramural
  • Review

MeSH terms

  • Animals
  • Cell Adhesion
  • Cell Movement / physiology*
  • Cell-Matrix Junctions / metabolism
  • Extracellular Matrix / metabolism*
  • Fibroblasts / physiology
  • Humans