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, 1010, 95-109

Lentiviral-mediated Gene Transfer of siRNAs for the Treatment of Huntington's Disease

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Lentiviral-mediated Gene Transfer of siRNAs for the Treatment of Huntington's Disease

Karine Cambon et al. Methods Mol Biol.

Abstract

This chapter describes the potential use of viral-mediated gene transfer in the central nervous system for the silencing of gene expression using RNA interference in the context of Huntington's disease (HD). Protocols provided here describe the design of small interfering RNAs, their encoding in lentiviral vectors (LVs) and viral production, as well as procedures for their stereotaxic injection in the rodent brain.

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