The soybean aphid (SA: Aphis glycines Matsumura) is a worldwide pest of soybean (Glycine max [L.] Merr.). The objectives of this study were to identify the type of aphid resistance and the resistance phenotype in soybean line 'P203', and to map the relative position of the gene involved. Compared with cultivars 'P746' and 'Dongnong 47', P203 was demonstrated to possess antixenosis resistance. P203 prevented aphids from reproducing in a choice test, but the resistance level decreased significantly in a no-choice test at 11 and 21 days after infestation. Analysis of 273 Dongnong 47/P203 F2 plants and confirmed using 260 F2:3 families revealed that a single dominant gene from P203 was positioned between marker loci Sat_377 and Satt409 on chromosome 8. The gene was further mapped to a 1.57 Mb interval flanked by marker loci BARCSOYSSR_08_1451 and BARCSOYSSR_08_1527. We developed five new SSR markers in the target interval and the resistance locus mapped between new markers SSR_08_75 and SSR_08_88 with the genetic distance of 1.1 and 1.0 cM corresponding to a physical distance of 192 kb on the Williams 82 8X draft genome assembly (Glyma1.01). A single serine/threonine protein kinase gene is present in this region, suggesting that the SA resistance mechanism in P203 may be different from those previously reported. Therefore, the resistance gene could very well be novel, and could be valuable in soybean aphid resistance breeding programs.