As the recalcitrance of biofilm-mediated infections to the anti-infective treatment has an adverse effect on patient's health, the main objective of this study was to investigate the capacity of clinical isolates of Staphylococcus aureus with different resistance patterns to form biofilms. S. aureus strains are among the most representative etiology of infections in the health-care environment of Milad hospital in Iran. The results showed that out of 80 analyzed strains, 27 methicillin resistant S. aureus (MRSA) and 29 methicillin susceptible S. aureus (MSSA) were positive for biofilm development ability, without any significant correlation observed between MRSA and biofilm production.