Invasive African Salmonella Typhimurium induces bactericidal antibodies against O-antigens

Microb Pathog. 2013 Oct;63:19-23. doi: 10.1016/j.micpath.2013.05.014. Epub 2013 Jun 10.

Abstract

Nontyphoidal Salmonella are a major and emerging cause of fatal invasive disease in Africa, and are genetically distinct from those found elsewhere in the world. Understanding the targets of protective immunity to these African Salmonellae is key to vaccine development. We immunized mice and rabbits with heat-inactivated wild-type African invasive Salmonella Typhimurium D23580 and rough mutants lacking O-antigen. Wild-type Salmonella, unlike rough bacteria, induced a large bactericidal antibody response mainly against O-antigen. Bactericidal ability of anti-O-antigen antibodies was confirmed following purification by affinity chromatography. The current findings support the development of an O-antigen conjugate vaccine against invasive nontyphoidal Salmonellae for Africa.

Keywords: Conjugate vaccine; NTS; Nontyphoidal Salmonella; O-antigen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Bacterial / blood*
  • Blood Bactericidal Activity*
  • Humans
  • Mice
  • O Antigens / immunology*
  • Rabbits
  • Salmonella Infections / immunology*
  • Salmonella Infections / microbiology*
  • Salmonella Vaccines / administration & dosage
  • Salmonella Vaccines / immunology*
  • Salmonella typhimurium / immunology*
  • Salmonella typhimurium / isolation & purification
  • Vaccination / methods

Substances

  • Antibodies, Bacterial
  • O Antigens
  • Salmonella Vaccines