R-loops in bacterial transcription: their causes and consequences

Transcription. Jul-Aug 2013;4(4):153-7. doi: 10.4161/trns.25101. Epub 2013 Jun 11.

Abstract

Nascent untranslated transcripts in bacteria are prone to generating RNA-DNA hybrids (R-loops); Rho-dependent transcription termination acts to reduce their prevalence. Here we discuss the mechanisms of R-loop formation and growth inhibition in bacteria.

Keywords: NusG; R-loop; Rho; backtracking; transcription termination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / genetics
  • Bacteria / metabolism*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • DNA Replication
  • DNA, Bacterial / metabolism*
  • DNA-Directed RNA Polymerases / metabolism
  • Peptide Elongation Factors / genetics
  • Peptide Elongation Factors / metabolism
  • RNA, Bacterial / metabolism*
  • Rho Factor / genetics
  • Rho Factor / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcription, Genetic

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Peptide Elongation Factors
  • RNA, Bacterial
  • Rho Factor
  • Transcription Factors
  • DNA-Directed RNA Polymerases