The complexity of identifying cancer stem cell biomarkers

Cancer Invest. 2013 Jul;31(6):404-11. doi: 10.3109/07357907.2013.802800. Epub 2013 Jun 11.


The efficacy of glioma therapy can be considerably improved if it eliminates cancer stem cells (CSCs); however, to achieve this, CSCs markers are required. This study investigated the influence of micro-environmental changes on CSCs in hypoxic, serum deprived U87-MG and the corresponding control cells. Proteomic analysis produced a wide dataset, depicting the changes that occur at the proteomic level in the differentiated and undifferentiated U87-MG cell line. With the IPA analysis, HPRD and literature reviews, 11 proteins were proposed as potential differentiated biomarkers for CSCs namely Hsp90β1, vimentin, PGK1, GAPDH, EIF4e, TPI1, HspA8, HNRNPK, NAMPT, CCSNK2A1, and ANXA2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AC133 Antigen
  • Antigens, CD / metabolism
  • Biomarkers, Tumor / metabolism*
  • Cell Hypoxia
  • Cell Line, Tumor
  • Cell Shape
  • Culture Media, Serum-Free
  • Glioma
  • Glycoproteins / metabolism
  • Humans
  • Neoplastic Stem Cells / metabolism*
  • Octamer Transcription Factor-3 / metabolism
  • Peptides / metabolism
  • Proteome / metabolism
  • Tumor Microenvironment
  • Up-Regulation
  • Vimentin / metabolism


  • AC133 Antigen
  • Antigens, CD
  • Biomarkers, Tumor
  • Culture Media, Serum-Free
  • Glycoproteins
  • Octamer Transcription Factor-3
  • POU5F1 protein, human
  • Peptides
  • Proteome
  • Vimentin