6-Methylsulfinylhexyl isothiocyanate modulates endothelial cell function and suppresses leukocyte adhesion

J Nat Med. 2014 Jan;68(1):144-53. doi: 10.1007/s11418-013-0784-x. Epub 2013 Jun 12.


6-Methylsulfinylhexyl isothiocyanate (6-MSITC) is an active compound in wasabi (Wasabia japonica Matsum.), which is one of the most popular spices in Japan. 6-MSITC suppresses lipopolysaccharide-induced macrophage activation, arachidonic- or adenosine diphosphate-induced platelet activation, and tumor cell proliferation. These data indicate that 6-MSITC has several biological activities involving anti-inflammatory, anti-coagulant, and anti-apoptosis properties. Endothelial cells (ECs) maintain vascular homeostasis and play crucial roles in crosstalk between blood coagulation and vascular inflammation. In this study, we determined the anti-coagulant and anti-inflammatory effects of 6-MSITC on human umbilical vein endothelial cells (HUVECs). 6-MSITC slightly reduced tissue factor expression, but did not alter von Willebrand factor release in activated HUVECs. 6-MSITC modulated the generation of activated protein C, which is essential for negative regulation of blood coagulation, on normal ECs. In addition, 6-MSITC reduced tumor necrosis factor-α (TNF-α)-induced interleukin-6 and monocyte chemoattractant protein-1 expression. 6-MSITC markedly attenuated TNF-α-induced adhesion of human monoblast U937 cells to HUVECs and reduced vascular cell adhesion molecule-1 and E-selectin mRNA expression in activated ECs. These results showed that 6-MSITC modulates EC function and suppresses cell adhesion. This study provides new insight into the mechanism of the anti-inflammatory effect of 6-MSITC, suggesting that 6-MSITC has therapeutic potential as a treatment for vasculitis and vascular inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents / pharmacology*
  • Anticoagulants / pharmacology*
  • Blood Coagulation / drug effects
  • Cell Adhesion / drug effects*
  • Chemokine CCL2 / metabolism
  • Dose-Response Relationship, Drug
  • E-Selectin / genetics
  • E-Selectin / metabolism
  • Human Umbilical Vein Endothelial Cells / cytology
  • Human Umbilical Vein Endothelial Cells / drug effects*
  • Human Umbilical Vein Endothelial Cells / metabolism
  • Humans
  • Interleukin-6 / metabolism
  • Isothiocyanates / pharmacology*
  • Leukocytes / cytology
  • Leukocytes / drug effects*
  • Leukocytes / metabolism
  • Protein C / metabolism
  • RNA, Messenger / metabolism
  • Thromboplastin / metabolism
  • Tumor Necrosis Factor-alpha / metabolism
  • U937 Cells
  • Vascular Cell Adhesion Molecule-1 / genetics
  • Vascular Cell Adhesion Molecule-1 / metabolism
  • von Willebrand Factor / metabolism


  • 6-methylsulfinylhexyl isothiocyanate
  • Anti-Inflammatory Agents
  • Anticoagulants
  • CCL2 protein, human
  • Chemokine CCL2
  • E-Selectin
  • IL6 protein, human
  • Interleukin-6
  • Isothiocyanates
  • Protein C
  • RNA, Messenger
  • SELE protein, human
  • Tumor Necrosis Factor-alpha
  • Vascular Cell Adhesion Molecule-1
  • von Willebrand Factor
  • Thromboplastin