Alterations of plasma immunoreactive glucagon-like peptide-1 behavior in non-insulin-dependent diabetics

Diabetes Res Clin Pract. May-Jun 1990;9(2):179-85. doi: 10.1016/0168-8227(90)90110-f.

Abstract

The basal level of plasma immunoreactive glucagon-like peptide-1 (IR GLP-1) was significantly elevated in non-insulin-dependent diabetics (NIDD), and this elevation of IR GLP-1 was mainly due to an increase in the large component of IR GLP-1, corresponding to the pancreatic form. During the oral glucose-tolerance test (OGTT), the total plasma IR GLP-1 decreased in normal subjects but increased significantly in diabetic patients. Chromatographic analysis showed that IR GLP-1 consisted of several different molecular forms. OGTT caused a decrease in the pancreatic form but increased the intestinal form in normal subject, resulting into a net decrease in total plasma IR GLP-1. Whereas in NIDD the increase in the intestinal form was more prominent and the suppression of the pancreatic form was practically abolished to result in a net increase of total plasma IR GLP-1. This observation is consistent with the fact that in normal subjects the total change in IR GLP-1 was significantly correlated with both the total change of gut glucagon as well as that of pancreatic glucagon, but in diabetics the total change of GLP-1 only correlated to that of gut glucagon. The impaired suppression of pancreatic GLP-1 and enhanced release of intestinal GLP-1 could have some physiological importance in NIDD.

Publication types

  • Comparative Study

MeSH terms

  • Blood Glucose / metabolism
  • Diabetes Mellitus, Type 2 / blood*
  • Glucagon / blood*
  • Glucagon-Like Peptide 1
  • Glucose Tolerance Test
  • Humans
  • Middle Aged
  • Peptide Fragments / blood*
  • Protein Precursors / blood*
  • Radioimmunoassay
  • Reference Values

Substances

  • Blood Glucose
  • Peptide Fragments
  • Protein Precursors
  • Glucagon-Like Peptide 1
  • Glucagon