Promoting Myelination in an in Vitro Mouse Model of the Peripheral Nervous System: The Effect of Wine Ingredients [Corrected]

PLoS One. 2013 Jun 7;8(6):e66079. doi: 10.1371/journal.pone.0066079. Print 2013.

Abstract

Protective properties of moderate wine consumption against cancers, cardiovascular, metabolic and degenerative diseases have been reported in various clinical studies. Here, we analysed the effect of red wine (RW) and white wine (WW) on myelination using an in vitro embryonic co-culture mouse model. The total amount of myelin was found to be significantly increased after RW and WW treatment, while only RW significantly increased the number of internodes. Both types of wine increased rat Schwann cell- (rSC) expression of the NAD+-dependent deacetylase sirtuin-two-homolog 2 (Sirt2), a protein known to be involved in myelination. Detailed chemical analysis of RW revealed a broad spectrum of anthocyanins, piceids, and phenolics, including resveratrol (RSV). In our assay system RSV in low concentrations induced myelination. Furthermore RSV raised intracellular glutathione concentrations in rSCs and in co-cultures and therefore augmented antioxidant capacity. We conclude that wine promotes myelination in a rodent in vitro model by controlling intracellular metabolism and SC plasticity. During this process, RSV exhibits protective properties; however, the fostering effect on myelinaton during exposure to wine appears to be a complex interaction of various compounds.

MeSH terms

  • Animals
  • Coculture Techniques
  • Ganglia, Spinal / cytology
  • Ganglia, Spinal / drug effects
  • Ganglia, Spinal / metabolism
  • Glutathione / metabolism
  • Immunohistochemistry
  • Mice
  • Mice, Inbred C57BL
  • Models, Animal
  • Myelin Sheath / drug effects
  • Myelin Sheath / metabolism*
  • Peripheral Nerves / cytology
  • Peripheral Nerves / drug effects
  • Peripheral Nerves / metabolism*
  • Rats
  • Resveratrol
  • Schwann Cells / cytology
  • Schwann Cells / drug effects
  • Schwann Cells / metabolism
  • Sirtuin 1 / metabolism
  • Sirtuin 2 / metabolism
  • Stilbenes / pharmacology
  • Wine*

Substances

  • Sirt2 protein, rat
  • Stilbenes
  • Sirt1 protein, rat
  • Sirtuin 1
  • Sirtuin 2
  • Glutathione
  • Resveratrol

Grant support

No current external funding sources for this study.