This review highlights the current status on the synthesis and applications of mesoporous silica spherical particles. Mesoporous silica spherical particles are expected to be applied in various fields and the properties depend on the size and size distribution. Many researchers have been working on the synthesis of mesoporous silica spherical particles by means of several approaches and a variety of synthetic parameters have been correlated to the nature of the products. The synthetic approaches are categorized into following four; emulsion based approach, formation from a homogeneous solution, aerosol and morphology template. In this review, the preparation and the potential applications of mesoporous silica spherical particles are summarized.