3D structural patterns in scalable, elastomeric scaffolds guide engineered tissue architecture

Adv Mater. 2013 Aug 27;25(32):4459-65. doi: 10.1002/adma.201301016. Epub 2013 Jun 14.

Abstract

Microfabricated elastomeric scaffolds with 3D structural patterns are created by semiautomated layer-by-layer assembly of planar polymer sheets with through-pores. The mesoscale interconnected pore architectures governed by the relative alignment of layers are shown to direct cell and muscle-like fiber orientation in both skeletal and cardiac muscle, enabling scale up of tissue constructs towards clinically relevant dimensions.

Keywords: alignment; heart; microfabrication; muscle; polyglycerol sebacate (PGS).

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Line
  • Decanoates / chemistry*
  • Elastomers
  • Glycerol / analogs & derivatives*
  • Glycerol / chemistry
  • Mice
  • Microtechnology
  • Myoblasts / cytology*
  • Myocardium / cytology*
  • Polymers / chemistry*
  • Porosity
  • Rats
  • Tissue Engineering / methods*
  • Tissue Scaffolds / chemistry*

Substances

  • Decanoates
  • Elastomers
  • Polymers
  • elastomeric polymer
  • poly(glycerol-sebacate)
  • Glycerol