Attenuated Salmonella enterica serovar Typhimurium lacking the ZnuABC transporter: an efficacious orally-administered mucosal vaccine against salmonellosis in pigs

Vaccine. 2013 Aug 12;31(36):3695-701. doi: 10.1016/j.vaccine.2013.05.105. Epub 2013 Jun 13.


We have recently demonstrated that an attenuated strain of Salmonella enterica serovar Typhimurium unable to synthesize the zinc transporter ZnuABC (S. Typhimurium ΔznuABC), is able to protect mice against systemic and enteric salmonellosis and is safe in pigs. Here, we have tested the protective effects of S. Typhimurium ΔznuABC in pigs. Resistance to challenge with the fully virulent strain S. Typhimurium ATCC 14028 was assessed in animals vaccinated with S. Typhimurium ΔznuABC (two dosages tested), in controls vaccinated with a formalin-inactivated virulent strain and in unvaccinated controls. Clinical signs of salmonellosis, faecal shedding and bacterial colonization of organs were used to assess vaccine-induced protection. After the challenge, pigs vaccinated with the attenuated S. Typhimurium ΔznuABC strain did not display clinical signs of salmonellosis (fever or diarrhoea). The vaccine also reduced intestinal tract colonization and faecal shedding of the fully virulent Salmonella strain, as compared to control groups. S. Typhimurium ΔznuABC represents a promising candidate vaccine against salmonellosis in pigs.

Keywords: Mucosal vaccine; Oral delivery; Pigs; S. Typhimurium ΔznuABC; Salmonella; Salmonellosis; Vaccine.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Bacterial Proteins / administration & dosage
  • Bacterial Proteins / immunology*
  • Bacterial Shedding
  • Cation Transport Proteins / administration & dosage
  • Cation Transport Proteins / immunology*
  • Feces / microbiology
  • Female
  • Immunity, Mucosal
  • Interferon-gamma / immunology
  • Mouth Mucosa / immunology
  • Salmonella Infections, Animal / prevention & control*
  • Salmonella Vaccines / administration & dosage
  • Salmonella Vaccines / immunology*
  • Salmonella typhimurium / immunology
  • Salmonella typhimurium / pathogenicity
  • Sus scrofa
  • Tumor Necrosis Factor-alpha / immunology
  • Vaccines, Attenuated / administration & dosage
  • Vaccines, Attenuated / immunology
  • Virulence


  • Bacterial Proteins
  • Cation Transport Proteins
  • Salmonella Vaccines
  • Tumor Necrosis Factor-alpha
  • Vaccines, Attenuated
  • Interferon-gamma