Regulation of iron transport related genes by boron in the marine bacterium Marinobacter algicola DG893

Metallomics. 2013 Aug;5(8):1025-30. doi: 10.1039/c3mt00068k.

Abstract

While there has been extensive interest in the use of boron isotope ratios as a surrogate of pH in paleoclimate studies in the context of climate change-related questions, the high (0.4 mM) concentration and the depth-independent (conservative or non-nutrient-like) concentration profile of this element have led to boron being neglected as a potentially biologically relevant element in the modern ocean. Here we report that boron affects the expression of a number of protein and genes in the "algal-associated" Gram-negative marine bacterium Marinobacter algicola DG893. Most intriguingly, a number of these proteins and genes are related to iron uptake. In a recent separate publication we have shown that boron regulates one such iron transport related protein, i.e. the periplasmic iron binding protein FbpA via a direct interaction of the metalloid with this protein. Here we show that a number of other iron uptake related genes are also affected by boron but in the opposite way i.e. they are up-regulated. We propose that the differential effect of boron on FbpA expression relative to other iron transport related genes is a result of an interaction between boron and the global iron regulatory protein Fur.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / metabolism
  • Boron / chemistry*
  • Gene Expression Profiling
  • Gene Expression Regulation, Bacterial*
  • Hydrogen-Ion Concentration
  • Iron / metabolism*
  • Marinobacter / genetics*
  • Marinobacter / metabolism*
  • Periplasmic Binding Proteins / metabolism
  • Proteome
  • Repressor Proteins / metabolism
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Bacterial Proteins
  • Periplasmic Binding Proteins
  • Proteome
  • Repressor Proteins
  • ferric uptake regulating proteins, bacterial
  • Iron
  • Boron