WUSCHEL-responsive At5g65480 interacts with CLAVATA components in vitro and in transient expression

PLoS One. 2013 Jun 11;8(6):e66345. doi: 10.1371/journal.pone.0066345. Print 2013.


The CLAVATA (CLV) signaling pathway is essential for shoot meristem homeostasis in Arabidopsis. CLV acts to limit the expression domain of the stem cell-promoting gene WUSCHEL (WUS). The closely related receptor-kinases CLV1 and BAM1 are key components in this pathway; however, the downstream factors that link the receptors to WUS regulation are poorly understood. The Arabidopsis gene At5g65480 was recently identified as a direct transcriptional target up-regulated by WUS. We have independently identified this gene which we term CCI1 as a CLV1 and BAM1 interacting protein in vitro and in transient expression. CCI1 has phosphatidylinositide-binding activity in vitro and localizes to the plasma membrane in transient expression. Furthermore, CLV signaling components and CCI1 both partition to detergent-resistant membrane microdomains characterized as lipid rafts.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Gene Expression Regulation, Plant / genetics
  • Gene Expression Regulation, Plant / physiology
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism*
  • Signal Transduction / genetics
  • Signal Transduction / physiology


  • Arabidopsis Proteins
  • Homeodomain Proteins
  • WUSCHEL protein, Arabidopsis