[Effect of Mn(II) on the error-prone DNA polymerase iota activity in extracts from human normal and tumor cells]

Mol Gen Mikrobiol Virusol. 2013:(1):14-20.
[Article in Russian]


The DNA polymerase iota (Pol iota), which has some peculiar features and is characterized by an extremely error-prone DNA synthesis, belongs to the group of enzymes preferentially activated by Mn2+ instead of Mg2+. In this work, the effect of Mn2+ on DNA synthesis in cell extracts from a) normal human and murine tissues, b) human tumor (uveal melanoma), and c) cultured human tumor cell lines SKOV-3 and HL-60 was tested. Each group displayed characteristic features of Mn-dependent DNA synthesis. The changes in the Mn-dependent DNA synthesis caused by malignant transformation of normal tissues are described. It was also shown that the error-prone DNA synthesis catalyzed by Pol iota in extracts of all cell types was efficiently suppressed by an RNA aptamer (IKL5) against Pol iota obtained in our work earlier. The obtained results suggest that IKL5 might be used to suppress the enhanced activity of Pol iota in tumor cells.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Aptamers, Nucleotide / pharmacology
  • Cell Line, Tumor / drug effects
  • Cell Line, Tumor / enzymology
  • Cell Transformation, Neoplastic / genetics
  • DNA / biosynthesis
  • DNA Polymerase iota
  • DNA-Directed DNA Polymerase / genetics*
  • DNA-Directed DNA Polymerase / metabolism*
  • Dose-Response Relationship, Drug
  • HL-60 Cells / drug effects
  • Humans
  • Manganese / pharmacology*
  • Melanoma / drug therapy
  • Melanoma / enzymology*
  • Mice
  • Mice, Inbred C57BL
  • Uveal Neoplasms / drug therapy
  • Uveal Neoplasms / enzymology*


  • Aptamers, Nucleotide
  • Manganese
  • DNA
  • DNA-Directed DNA Polymerase
  • DNA Polymerase iota

Supplementary concepts

  • Uveal melanoma